Phos-tag鈩 Acrylamide

SDS-PAGE 鍒嗙涓嶅悓纾烽吀鍖栨按骞崇殑铔嬬櫧锛?/strong>

銆€銆€鍦ㄤ笉浣跨敤鏀惧皠鎬у悓浣嶇礌鐨勬儏鍐典笅锛屽埄鐢 Phos-tag鈩 SDS-PAGE 鍗冲彲鍒嗙涓嶅悓鏉″甫涓殑纾烽吀鍖栧拰闈炵７閰稿寲铔嬬櫧銆傚垎绂诲悗鐨勫嚌鑳跺彲鐢ㄤ簬 Western blotting 鍜岃川璋卞垎鏋愮瓑鍚庣画瀹為獙銆侟/span>

銆€銆€Phos-tag鈩 SDS-PAGE 鎿嶄綔绠€鍗曪紝鍙渶鍦ㄥ父瑙 SDS-PAGE 鑳朵腑鍔犲叆 Phos-tag鈩 Acrylamide 鍜 Mn²⁺ 鎴栬€ Zn²⁺ 鍗冲彲杩涜瀹為獙銆傚湪鐢垫吵杩囩▼涓紝纾烽吀鍖栬泲鐧界殑纾烽吀鍩哄洟涓 Phos-tag鈩 涓殑浜屼环閲戝睘绂诲瓙鐩哥粨鍚堬紝闄嶄綆鍏惰縼绉婚€熷害锛屼粠鑰屽彲鍖哄垎纾烽吀鍖栦笌闈炵７閰稿寲铔嬬櫧銆侟/span>

鈼咟/span>鍘熺悊

鈼咟/span>浼樼偣銆佺壒鑹秉/span>

鈼廃span style="margin: 0px; padding: 0px; font-family: "Times New Roman";">  閲囩敤 Phos-tag鈩 SDS-PAGE 鍙交鏉惧垎绂荤７閰稿寲铔嬬櫧

銆€  鏃犱换浣曟斁灏勬€у厓绱犲強鍖栧鏍囪锛?/strong>

鈼廃/span>  鍙娴嬩笉鍚岀７閰稿寲姘村钩鐨勭７閰稿寲铔嬬櫧

銆€  鏃犻渶浠讳綍纾烽吀鍖栨姉浣擄紒

鈼廃/span>  閫傜敤浜嶞span style="margin: 0px; padding: 0px; color: rgb(192, 0, 0);">鍐呮簮鎬ц泲鐧键/strong>鐨勭７閰稿寲鍒嗘瀽锛?/span>

鈼咟/span>妗堜緥銆佸簲鐢?/span>

銆愪娇鐢≒hos-tag鈩 SDS-PAGE鐨勭７閰稿寲/闈炵７閰稿寲铔嬬櫧姣旇緝銆慄/span>

鎴戞帹鑽愪娇鐢≒hos-tag 鈩 鈥斺€斾笢浜ぇ瀛︾爺绌堕櫌鍖诲鐮旂┒绉 灏忓窛瑙変箣

銆€銆€Phos-tag 鈩 鏄笓涓虹爺绌剁７閰稿寲铔嬬櫧鑰屾柊寮€鍙戝嚭鏉ョ殑璇曞墏銆傛浜у搧浣跨敤鏂逛究锛屼笉浣嗗彲鐢ㄤ簬浣撳瀹為獙锛岃繕鑳藉畾閲忓垎鏋愪綋鍐呰泲鐧界殑纾烽吀鍖栨按骞炽€侾hos-tag 鈩 SDS-PAGE 鍙敤浜庡父瑙勭數娉冲疄楠岋紝鏃犻渶璐拱鐗规畩璁惧锛屾€т环姣旈珮銆備紶缁熻泲鐧界７閰稿寲鐨勭爺绌堕渶瑕佺壒寮傜殑纾烽吀鍖栨姉浣撱€丷I 绛夊叾瀹冭瘯鍓傦紝鎿嶄綔澶嶆潅锛岃姳璐瑰ぇ锛屼笖鏀惧皠鎬у厓绱犱細鏈夊畨鍏ㄩ殣鎮ｏ紝鑰 Phos-tag 鈩 鐨勫嚭鐜版伆鎭板彲浠ュ讥琛ヨ繖浜涚己鐐癸紝涓虹７閰稿寲铔嬬櫧鐮旂┒鎻愪緵鏂扮殑鏂瑰悜銆侟/span>

纾烽吀鍖栬泲鐧藉拰闈炵７閰稿寲铔嬬櫧鍒╃敤Phos-tag 鈩 SDS-PAGE 鐨勫垎绂绘晥鏋滃浘

Lane 1 涓洪潪纾烽吀鍖栬泲鐧斤紝Lane 2-5 涓虹７閰稿寲铔嬬櫧锛屽悇铔嬬櫧鍥犵７閰稿寲鐘舵€佷笉鍚岃€屾潯甯﹁縼绉荤巼涔熸湁鎵€涓嶅悓銆侟/span>

銆€銆€纾烽吀鍖? 闈炵７閰稿寲铔嬬櫧鐨勬暟閲忔瘮銆佺７閰稿寲绋嬪害銆佺７閰稿寲铔嬬櫧鐨勪赴搴︾瓑閮藉彲鏍规嵁鏉″甫杩佺Щ鍜屾潯甯︽祿搴︽眰寰椼€侟/span>

銆愯祫鏂欐彁渚涖€慄/span>

鏃ユ湰涓滀含澶у鐮旂┒鐢熼櫌鍖诲绯荤爺绌剁

銆愪簩缁寸數娉充腑鐨勫簲鐢細鍒嗘瀽 hnRNP K 纾烽吀鍖栧紓鏋勪綋銆慄/span>

銆€銆€灏忛紶宸ㄥ櫖缁嗚優 J774.1 缁 LPS 鍒烘縺鍚庯紝瑁傝В缁嗚優锛岀粡杩囧厤鐤矇娣€娉曞垎绂诲緱鍒 hnRNP K 銆傚湪浜岀淮鐢垫吵涓紝涓€缁存槸IPG 鑳讹紝浜岀淮鏄 Phos-tag 鈩 SDS-PAGE锛屽彲鍒嗙 hnRNP K 鐨勫紓鏋勪綋銆傚埄鐢ㄨ川璋变华锛屽彲浠ョ‘璁や笉鍚岀殑鐐逛唬琛ㄤ笉鍚岀殑浜氬瀷鎴栦慨楗拌泲鐧姐€侟/span>

浜岀淮鐢垫吵

鍚屼竴涓瓑鐢电偣鐨勪綅缃笂锛屼笉鍚屼綅鐐瑰彂鐢熺７閰稿寲閮藉彲浠ヨ鍖哄垎寮€鏉ワ紙渚婜 spots 6 vs. 8 and spots 4 vs. 7锛堻/span>

銆愬弬鑰冩枃鐚€慄/span>

Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate-affinity electrophoresis. Y. Kimura, K. Nagata, N Suzuki, R. Yokoyama, Y. Yamanaka, H. Kitamura, H. Hirano, and O. Ohara, Proteomics , Nov 2010; 10(21): 3884-95.

銆愮粨鏋滄彁渚涖€慄/span>

銆€銆€妯花甯傜珛澶у 鐢熷懡绾崇背绯荤粺绉戝鐮旂┒绉 鐢熺墿浣撹秴鍒嗗瓙绯荤粺绉戝涓撲笟 鏈ㄦ潙寮ョ敓锛圖r. Y. Kimura锛夈€佸钩閲庝箙锛圖r. H. Hirano锛堻/span>鐞嗗寲瀛︾爺绌舵墍 RCAI 灏忓師鏀迭/span>

銆怑GF 鍒烘縺鍓嶅悗MAPK 纾烽吀鍖栨按骞崇殑鍙樺寲銆慄/span>

銆€銆€甯歌 SDS-PAGE 鍜孭hos-tag^TM SDS-PAGE 鍚庤繗琛屽厠鐤嵃杩瑰疄楠屽垎鏋 EGF 鍒烘縺鐨 A431 缁嗚優涓 MAPK 纾烽吀鍖栨按骞炽€侟/span>

鎽樿嚜 Kinoshita-Kikuta, E. et al., Mol.Cell. Proteomics. (2007)6: 356.