• 鏈€楂樻晥鐨勫箍璋盌NA杞煋璇曞墏

  
  

  
  

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閫傚悎鎮ㄧ爺绌剁殑涓€娴丏NA杞煋璇曞墏

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缁忓墠鍑犱唬FuGENE^庐鎶€鏈敼杩涘悗鐨凢uGENE^庐 4K鑳藉灏咲NA楂樻晥閫掗€佽嚦鍚勭缁嗚優绯讳腑锛屽悓鏃跺疄鐜颁綘鎵€鏈熷緟鐨凢uGENE^庐璇曞墏鐨勬俯鍜屻€佷綆姣掓€ц浆鏌撱€傝幏寰楁瘮绔炰簤瀵规墜璇曞墏鏇撮珮鐨勮浆鏌撴晥鐜囷紝鍚屾椂鏈€澶ч檺搴﹀噺灏戝缁嗚優鍋ュ悍鐨勫奖鍝嶏紝杩涜€屽湪杞煋瀹為獙涓疄鐜版晥鐩婃渶澶у寲銆侟/span>

璇曞墏涓嶅惈鍔ㄧ墿鎴愬垎锛屽叾鍦℉EK293鍜孋HO缁嗚優涓殑寮哄ぇ鎬ц兘浣縁uGENE^庐 4K鎴愪负鐢熶骇铔嬬櫧璐ㄥ拰鐥呮瘨鐨勭悊鎯抽€夋嫨銆傛搷浣滅畝渚挎湁鍔╀簬鍦ㄥ疄鏂芥渶灏戜紭鍖栨帾鏂界殑鎯呭喌涓嬩篃鑳借幏寰楁渶浣崇粨鏋滐紝浠庤€屼负鎮ㄨ妭鐪佸疂璐电殑瀹為獙鏃堕棿銆侟/span>

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FuGENE^庐 4K璇曞墏鍏锋湁鏇村ソ鐨凞NA杞煋鎬ц兘锛屽悓鏃跺缁嗚優鍋ュ悍鐨勫奖鍝嶆渶灏廃/span>銆侟/span>浣跨敤FuGENE^庐 4K鎴朙ipofectamine鈩 3000锛圱hermoFisher Scientific锛夎繘琛屽姣斿疄楠岋紝A375銆丆HO-K1銆丠1299鍜孒CT116缁嗚優绯荤敤涓婅堪璇曞墏锛屾寜鐓т笉鍚岀殑杞煋璇曞墏锛欴NA姣斾緥锛岃浆鏌揋FP琛ㄨ揪杞戒綋銆?8灏忔椂鍚庯紝瀵归€忔槑搴曠粏鑳炲煿鍏绘澘涓粏鑳炵殑鎬籊FP鑽у厜鍊艰繘琛屾祴瀹氥€備娇鐢–ellTiter-Glo^庐 Luminescent Cell Viability Assay鍦ㄤ竴涓畬鍏ㄤ竴鏍风殑缁嗚優鍩瑰吇鏉夸笂娴嬪畾缁嗚優娲诲姏銆侟/span>

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